Probing which has an anti GFP antibody revealed some degradation of GFP Sec16B over the sample analysis. Immunofluorescence with your cells showed that suppression of Sec16A triggered a reduction of peripheral ERES (Sec31A labelling, Figure 3G in comparison with Figure 3F). Cell countCells were seeded in the same number and counted every 24 h for 4 days after transfection. Full-sized image View in article Introduction Introduction&bull. Western blotting shown that GFP Sec16B (~ 144 kD, #) additionally, the GFP control were bound efficiently towards GFP Trap®.
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